Catalase Test

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Catalase Test



5 years ago

~0.5 mins read

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This is a laboratory procedure used to differentiate staphylococci (catalase positive)  from  streptococci(catalase negative). 
  A catalase is a common enzyme found in nearly all living organisms exposed to oxygen.

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It catalyzes the decomposition of water and oxygen.

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It is important in protecting the cell from oxidative damage. 
 Procedure;
1.transfer a small amount of bacteria colony into a surface of clean, dry glass slide using a loop. 
2.place a drop of 3% hydrogen peroxide on the slide and mix. 
3.A positive result is the rapid evolution of oxygen(within 5-10sec) as evidenced by bubbling. 
4.A negative result no bubbles. 
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